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first strand cdna synthesis kit 2 0  (Genecopoeia)


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    Genecopoeia first strand cdna synthesis kit 2 0
    First Strand Cdna Synthesis Kit 2 0, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 96/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/first+strand+cdna+synthesis+kit+2+0/All-in-One+miRNA+First-Strand+cDNA+Synthesis+Kit+2%2E0+for+miRNA+qPCR+array/pm42073621-126-3-13
    Average 96 stars, based on 19 article reviews
    first strand cdna synthesis kit 2 0 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Reduced miR-99a-3p levels in systemic lupus erythematosus may promote B cell proliferation via NCAPG and the PI3K/AKT signaling pathway.
    Article Snippet: Background: Systemic lupus erythematosus is an immunologically dysregulated disease characterized by the presence of multiple autoantibodies.. In SLE, B lymphocytes contribute to the dysregulated production of autoantibodies and cytokines.. Recently, we discovered that miR-99a-3p binds to both EIF4EBP1 and NCAPG mRNA and that lowering miR-99a-3p can promote B cell autophagy in SLE by increasing EIF4EBP1 expression.

    Article Title: Prognostic Role of Uric Acid-Related Gene Signatures in Glioblastoma Multiforme: Insights from Bulk RNA and Single-Cell RNA Sequencing.
    Article Snippet: According to the manufacturer’s guidelines, the TRIzolTM Reagent (15596026CN, Invitrogen, Carlsbad, CA, USA) was used to extract ribonucleic acid (RNA) from clinical tissue specimens and cells cultured in the laboratory. .. The All-in-OneTM miRNA First-Strand cDNA Synthesis Kit 2.0 (Product Code QP113, produced by GeneCopoeia in Guangzhou, China) was utilized to transform the extracted ribonucleic acid (RNA) into complementary deoxyribonucleic acid (cDNA) via reverse transcription. .. Subsequently, the PowerTrackTM SYBR Green Master Mix, which is intended for quantitative polymerase chain reaction (qPCR) (Product Code A46109, provided by Thermo Fisher Scientific in the Waltham, MA, USA), was applied for quantification on the Roche LightCycler480 system (Product Code 900066, made by Roche in Basel, Switzerland).

    Article Title: Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism
    Article Snippet: Total RNA was extracted from fecal and colonic samples for miRNA analysis using RNAiso Plus (#9109, Takara). .. RNA was reverse-transcribed into miRNA cDNA synthesis using All-in-One miRNA First-Strand cDNA Synthesis Kit 2.0 (#QP114, GeneCopoeia, China). .. Quantification of miRNA cDNAs was conducted via real-time PCR using Hieff ® qPCR SYBR Green Master Mix (Low Rox Plus) (#11202ES08, GeneCopoeia, China) on ViiA 7 Real-Time PCR System (ABI, United States), following the manufacturer’s protocol.

    Article Title: Combined Diagnostic Value of Hsa-miR-592 and Hsa-miR-9-3p in Plasma for Methamphetamine Addicts.
    Article Snippet: Total RNA was obtained from a sample of 300 μL plasma utilizing TRIzol LS Reagent (Thermo Fisher Scientific, Waltham, MA, USA). .. All-in-OneTM miRNA First-Strand cDNA Synthesis Kit 2.0 (Gene Copoeia, Guangzhou, China) was used to reverse-transcribe into cDNA from 500 ng RNA. .. All qRT-PCR was carried out on the QuantSudio 7 Flex System (Applied Biosystems, Waltham, MA, USA) using the All-in-OneTM miRNA qRT-PCR Detection Kit (Gene Copoeia, Guangzhou, China).

    Article Title: Whole transcriptome sequencing identifies key lncRNAs, circRNAs and miRNAs in sepsis-associated acute lung injury.
    Article Snippet: The miRNA was isolated from lung tissues using the miRNA Isolation Kit (Omega Bio-Tek, China) based on the manufacturer’s instructions. .. The mouse miRNAs were reverse-transcribed into cDNA and qPCR detection was conducted using the All-in-One miRNA First-Strand cDNA Synthesis Kit 2.0 (QP113, Genecopoeia, United States) and ALL-in-One miRNA qPCR Kit (QP010, Genecopoeia, United States). .. Real-time PCR was performed on an ABI 7500 system (Thermo Fisher Scientific, Waltham, MA, United States).

    Article Title: Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism.
    Article Snippet: Total RNA was extracted from fecal and colonic samples for miRNA analysis using RNAiso Plus (#9109, Takara). .. RNA was reverse-transcribed into miRNA cDNA synthesis using All-in-One miRNA First-Strand cDNA Synthesis Kit 2.0 (#QP114, GeneCopoeia, China). .. Quantification of miRNA cDNAs was conducted via real-time PCR using Hieff® qPCR SYBR Green Master Mix (Low Rox Plus) (#11202ES08, GeneCopoeia, China) on ViiA 7 Real-Time PCR System (ABI, United States), following the manufacturer’s protocol.

    Article Title: Exosome‐delivered miR ‐410‐3p reverses epithelial‐mesenchymal transition, migration and invasion of trophoblasts in spontaneous abortion
    Article Snippet: For mRNA expression analysis, cDNA was synthesized with RevertAid First Strand cDNA Synthesis Kit (ThermoFisher, USA), and RNA isolation and quantitative real‐time PCR (RT‐PCR) was performed using PowerUpTM SYBRTM Green Master Mix (ThermoFisher). .. For miRNA expression analysis, reverse transcription was performed with All‐in‐OneTM miRNA First‐Strand cDNA Synthesis Kit 2.0 (GeneCopoeia, USA), and RT‐PCR was performed using All‐in‐OneTM miRNA qPCR Kit (GeneCopoeia). ..

    Article Title: Association of plasma microRNA-16-5p and abdominal aortic calcification in maintenance hemodialysis patients
    Article Snippet: .. Quantitative real-time polymerase chain reaction RNA was then reverse-transcribed using All-in-OneTM miRNA First-Strand cDNA Synthesis Kit 2.0(GeneCopoeia(Cat. No.QP113))according to manufacturer’s protocol. .. The expression levels of miR-16-5p were quantified with the Taq Pro Universal SYBR qPCR Master Mix(Vazyme(Cat.No.Q712)) according to manufacturer’s protocols by Roche LightCycler480 fluorescence quantitative PCR instrument. qRT-PCR reactions were carried out in total volume of 20ul containing 10ul 2 × Super Mix, 0.8 μL Primer(10 μM), 1 μL cDNA.

    Produced:

    Article Title: Prognostic Role of Uric Acid-Related Gene Signatures in Glioblastoma Multiforme: Insights from Bulk RNA and Single-Cell RNA Sequencing.
    Article Snippet: According to the manufacturer’s guidelines, the TRIzolTM Reagent (15596026CN, Invitrogen, Carlsbad, CA, USA) was used to extract ribonucleic acid (RNA) from clinical tissue specimens and cells cultured in the laboratory. .. The All-in-OneTM miRNA First-Strand cDNA Synthesis Kit 2.0 (Product Code QP113, produced by GeneCopoeia in Guangzhou, China) was utilized to transform the extracted ribonucleic acid (RNA) into complementary deoxyribonucleic acid (cDNA) via reverse transcription. .. Subsequently, the PowerTrackTM SYBR Green Master Mix, which is intended for quantitative polymerase chain reaction (qPCR) (Product Code A46109, provided by Thermo Fisher Scientific in the Waltham, MA, USA), was applied for quantification on the Roche LightCycler480 system (Product Code 900066, made by Roche in Basel, Switzerland).

    Reverse Transcription:

    Article Title: Prognostic Role of Uric Acid-Related Gene Signatures in Glioblastoma Multiforme: Insights from Bulk RNA and Single-Cell RNA Sequencing.
    Article Snippet: According to the manufacturer’s guidelines, the TRIzolTM Reagent (15596026CN, Invitrogen, Carlsbad, CA, USA) was used to extract ribonucleic acid (RNA) from clinical tissue specimens and cells cultured in the laboratory. .. The All-in-OneTM miRNA First-Strand cDNA Synthesis Kit 2.0 (Product Code QP113, produced by GeneCopoeia in Guangzhou, China) was utilized to transform the extracted ribonucleic acid (RNA) into complementary deoxyribonucleic acid (cDNA) via reverse transcription. .. Subsequently, the PowerTrackTM SYBR Green Master Mix, which is intended for quantitative polymerase chain reaction (qPCR) (Product Code A46109, provided by Thermo Fisher Scientific in the Waltham, MA, USA), was applied for quantification on the Roche LightCycler480 system (Product Code 900066, made by Roche in Basel, Switzerland).

    Article Title: Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism
    Article Snippet: Total RNA was extracted from fecal and colonic samples for miRNA analysis using RNAiso Plus (#9109, Takara). .. RNA was reverse-transcribed into miRNA cDNA synthesis using All-in-One miRNA First-Strand cDNA Synthesis Kit 2.0 (#QP114, GeneCopoeia, China). .. Quantification of miRNA cDNAs was conducted via real-time PCR using Hieff ® qPCR SYBR Green Master Mix (Low Rox Plus) (#11202ES08, GeneCopoeia, China) on ViiA 7 Real-Time PCR System (ABI, United States), following the manufacturer’s protocol.

    Article Title: Whole transcriptome sequencing identifies key lncRNAs, circRNAs and miRNAs in sepsis-associated acute lung injury.
    Article Snippet: The miRNA was isolated from lung tissues using the miRNA Isolation Kit (Omega Bio-Tek, China) based on the manufacturer’s instructions. .. The mouse miRNAs were reverse-transcribed into cDNA and qPCR detection was conducted using the All-in-One miRNA First-Strand cDNA Synthesis Kit 2.0 (QP113, Genecopoeia, United States) and ALL-in-One miRNA qPCR Kit (QP010, Genecopoeia, United States). .. Real-time PCR was performed on an ABI 7500 system (Thermo Fisher Scientific, Waltham, MA, United States).

    Article Title: Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism.
    Article Snippet: Total RNA was extracted from fecal and colonic samples for miRNA analysis using RNAiso Plus (#9109, Takara). .. RNA was reverse-transcribed into miRNA cDNA synthesis using All-in-One miRNA First-Strand cDNA Synthesis Kit 2.0 (#QP114, GeneCopoeia, China). .. Quantification of miRNA cDNAs was conducted via real-time PCR using Hieff® qPCR SYBR Green Master Mix (Low Rox Plus) (#11202ES08, GeneCopoeia, China) on ViiA 7 Real-Time PCR System (ABI, United States), following the manufacturer’s protocol.

    Article Title: Exosome‐delivered miR ‐410‐3p reverses epithelial‐mesenchymal transition, migration and invasion of trophoblasts in spontaneous abortion
    Article Snippet: For mRNA expression analysis, cDNA was synthesized with RevertAid First Strand cDNA Synthesis Kit (ThermoFisher, USA), and RNA isolation and quantitative real‐time PCR (RT‐PCR) was performed using PowerUpTM SYBRTM Green Master Mix (ThermoFisher). .. For miRNA expression analysis, reverse transcription was performed with All‐in‐OneTM miRNA First‐Strand cDNA Synthesis Kit 2.0 (GeneCopoeia, USA), and RT‐PCR was performed using All‐in‐OneTM miRNA qPCR Kit (GeneCopoeia). ..

    Article Title: Association of plasma microRNA-16-5p and abdominal aortic calcification in maintenance hemodialysis patients
    Article Snippet: .. Quantitative real-time polymerase chain reaction RNA was then reverse-transcribed using All-in-OneTM miRNA First-Strand cDNA Synthesis Kit 2.0(GeneCopoeia(Cat. No.QP113))according to manufacturer’s protocol. .. The expression levels of miR-16-5p were quantified with the Taq Pro Universal SYBR qPCR Master Mix(Vazyme(Cat.No.Q712)) according to manufacturer’s protocols by Roche LightCycler480 fluorescence quantitative PCR instrument. qRT-PCR reactions were carried out in total volume of 20ul containing 10ul 2 × Super Mix, 0.8 μL Primer(10 μM), 1 μL cDNA.

    Real-time Polymerase Chain Reaction:

    Article Title: Whole transcriptome sequencing identifies key lncRNAs, circRNAs and miRNAs in sepsis-associated acute lung injury.
    Article Snippet: The miRNA was isolated from lung tissues using the miRNA Isolation Kit (Omega Bio-Tek, China) based on the manufacturer’s instructions. .. The mouse miRNAs were reverse-transcribed into cDNA and qPCR detection was conducted using the All-in-One miRNA First-Strand cDNA Synthesis Kit 2.0 (QP113, Genecopoeia, United States) and ALL-in-One miRNA qPCR Kit (QP010, Genecopoeia, United States). .. Real-time PCR was performed on an ABI 7500 system (Thermo Fisher Scientific, Waltham, MA, United States).

    Article Title: Exosome‐delivered miR ‐410‐3p reverses epithelial‐mesenchymal transition, migration and invasion of trophoblasts in spontaneous abortion
    Article Snippet: For mRNA expression analysis, cDNA was synthesized with RevertAid First Strand cDNA Synthesis Kit (ThermoFisher, USA), and RNA isolation and quantitative real‐time PCR (RT‐PCR) was performed using PowerUpTM SYBRTM Green Master Mix (ThermoFisher). .. For miRNA expression analysis, reverse transcription was performed with All‐in‐OneTM miRNA First‐Strand cDNA Synthesis Kit 2.0 (GeneCopoeia, USA), and RT‐PCR was performed using All‐in‐OneTM miRNA qPCR Kit (GeneCopoeia). ..

    Article Title: Association of plasma microRNA-16-5p and abdominal aortic calcification in maintenance hemodialysis patients
    Article Snippet: .. Quantitative real-time polymerase chain reaction RNA was then reverse-transcribed using All-in-OneTM miRNA First-Strand cDNA Synthesis Kit 2.0(GeneCopoeia(Cat. No.QP113))according to manufacturer’s protocol. .. The expression levels of miR-16-5p were quantified with the Taq Pro Universal SYBR qPCR Master Mix(Vazyme(Cat.No.Q712)) according to manufacturer’s protocols by Roche LightCycler480 fluorescence quantitative PCR instrument. qRT-PCR reactions were carried out in total volume of 20ul containing 10ul 2 × Super Mix, 0.8 μL Primer(10 μM), 1 μL cDNA.

    Expressing:

    Article Title: Exosome‐delivered miR ‐410‐3p reverses epithelial‐mesenchymal transition, migration and invasion of trophoblasts in spontaneous abortion
    Article Snippet: For mRNA expression analysis, cDNA was synthesized with RevertAid First Strand cDNA Synthesis Kit (ThermoFisher, USA), and RNA isolation and quantitative real‐time PCR (RT‐PCR) was performed using PowerUpTM SYBRTM Green Master Mix (ThermoFisher). .. For miRNA expression analysis, reverse transcription was performed with All‐in‐OneTM miRNA First‐Strand cDNA Synthesis Kit 2.0 (GeneCopoeia, USA), and RT‐PCR was performed using All‐in‐OneTM miRNA qPCR Kit (GeneCopoeia). ..



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